A8 – Mike Heilemann & Erin Schuman

Quantitative mapping of synaptic proteins and mRNA in neurons & a nano-analytical platform to probe binding mechanisms of RNA-binding proteins

Synaptic activity is coupled to protein copy numbers, which vary and are regulated by the presence of mRNA and miRNA. We will develop a nano-scale imaging platform that will allow a comprehensive analysis of protein, mRNA and microRNA “localizome”, by visualizing multiple proteins and mRNA species in the same cell using single-molecule super-resolution imaging with large multiplexing capabilities. Next to localizing and quantifying protein and mRNA, we aim to develop tools that allow super resolution of molecules that regulate miRNA/mRNA, and newly transcribed proteins. In addition, we will develop a nano-analytical platform to study the mechanism of RNA-binding proteins binding to their target RNA sequence. This novel platform will allow the study of unmodified proteins, and provide information on binding equilibria and kinetics, sequence specificity, interaction with co-factors and the influence of mutations.

Phone

ORCID

Google Schoolar

+49 (0)69 – 798-29736

0000-0002-9821-3578

Heilemann

+49(0) 69 850033 1000

0000-0002-7053-1005

Schuman

Address

Institute of Physical and Theoretical Chemistry
Johann Wolfgang Goethe-University
Max-von-Laue-Str. 7

Building N120/124
60438 Frankfurt, Germany

Max Planck Institute for Brain Research
Max-von-Laue-Str. 4

L.1.021
60438 Frankfurt am Main
Germany

Co-Workers on RNA


Dr. Hans Dieter Barth

Postdoc

Email: h.d.barth@chemie.uni-frankfurt.de


Nina Deußner-Helfmann

PhD. Student

Email: deussner@chemie.uni-frankfurt.de


Ann-Christin Andres

PhD. Student

Email: andres@chemie.uni-frankfurt.de

Phone: +49(0) 69 798 29462